【实验专用】非洲爪蟾卵母细胞现货供应Xenopus oocytes — ready to ship

为何选择爪蟾卵母细胞Why Xenopus oocytes?

爪蟾卵母细胞直径约 1 mm，肉眼可见，易于显微操作。外源 mRNA（cRNA）注射后 2–4 天即可在细胞膜上高效表达目标蛋白，蛋白折叠与翻译后修饰与哺乳动物系统高度相近。天然膜背景极为纯净，内源离子通道电流极小，是研究膜蛋白结构与功能的金标准表达系统之一。Xenopus oocytes (~1 mm diameter) are easily handled under a dissecting scope. Exogenous mRNA injected into the cytoplasm yields robust membrane-protein expression within 2–4 days, with mammalian-like folding and post-translational modification. The endogenous membrane background is exceptionally clean — a gold-standard heterologous expression system.

主要应用领域Applications

• 基因克隆与蛋白功能验证：注射体外转录的 cRNA，快速获得目标蛋白在生理膜环境中的功能数据。Functional cloning & validation: inject in-vitro-transcribed cRNA to characterise target proteins in a native membrane context.
• 异源性离子通道 / 受体表达：表达来自哺乳动物、植物或其他生物的膜蛋白，结合双电极电压钳（TEVC）进行高精度电生理分析。Heterologous channel/receptor expression: express mammalian, plant or other membrane proteins; analyse with two-electrode voltage clamp (TEVC).
• 药物靶点筛选与机制研究：批量筛查候选化合物对离子通道或转运蛋白的调节作用，获取浓度–效应关系曲线。Drug target screening: screen compounds against channels/transporters; derive concentration–response curves.
• 转运蛋白功能测定：利用放射性同位素或荧光底物检测营养物质、离子的跨膜转运活性，定量评价转运动力学。Transporter assays: measure nutrient or ion flux using radioisotopes or fluorescent substrates; quantify transport kinetics.

供应与服务说明Supply & service notes

• 野生型与白化型两种品系可选：野生型产量大，白化型色素干扰低，利于荧光实验。Wild-type (high yield) or albino (low pigment, better fluorescence) — specify on order.
• 可提供带滤泡或胶原酶去滤泡处理的卵母细胞，需提前说明。Follicle-intact or collagenase-defolliculated — specify on order.
• 全程冷链配送，建议到货当日使用；如需注射与 TEVC 全流程服务，可直接委托本中心完成并交付电生理数据。Cold-chain delivery; use same day of arrival. Full-service injection + TEVC available — we deliver electrophysiology data directly.