首页Home / 爪蟾供应Xenopus Supply / 热带爪蟾成体X. tropicalis adults
热带爪蟾成体
热带爪蟾 · 进口繁殖群X. tropicalis · Imported breeding stock

进口繁殖成年热带爪蟾（雌 / 雄可选）Adult Xenopus tropicalis — male / female available

热带爪蟾（Xenopus tropicalis）是继非洲爪蟾（X. laevis）之后最重要的脊椎动物发育模型之一。与 X. laevis 不同，X. tropicalis 为二倍体基因组，基因组较小（约 1.7 Gb），世代周期仅 4–5 个月，是正向遗传筛选、基因组功能研究与 CRISPR 编辑的理想选择。本中心引进专业繁殖群，提供携带完整检疫证明的进口成体，雌雄均有现货。The western clawed frog (Xenopus tropicalis) is one of the most important vertebrate developmental models after X. laevis. Unlike X. laevis, X. tropicalis has a diploid genome (~1.7 Gb), with a generation time of only 4–5 months — ideal for forward genetic screens, genomic functional studies and CRISPR editing. We import animals from professional breeding facilities with full quarantine documentation.

参数Parameter详情Details
物种SpeciesXenopus tropicalis（热带爪蟾）
性别Sex雌 / 雄可选（专业鉴别）Female / male (professionally sexed)
体长Body length5–7 cm（性成熟个体sexually mature）
来源Source专业进口繁殖群，携带检疫证明Imported professional breeding colony, with quarantine certification
存活率保证Survival guarantee≥ 98%（到货 48 小时内within 48 h of arrival）
运输Shipping控温充氧包装，次日达（极端气温暂停邮寄）Temperature-controlled oxygenated packaging, next-day delivery

💡 热带爪蟾对温度敏感（最适 25–28℃），收到后请参考附赠养殖指南尽快完成水温适应，勿与 X. laevis（最适 18–22℃）混养。💡 X. tropicalis is temperature-sensitive (optimal 25–28 °C). On arrival, follow the included care guide for water temperature acclimation. Do not house together with X. laevis (optimal 18–22 °C).

询价与预约Enquire & reserve 非洲爪蟾成体X. laevis adults

热带爪蟾 vs 非洲爪蟾：如何选择？X. tropicalis vs X. laevis: which to choose?

两种爪蟾各有优势，科研人员应根据实验目标选择合适的物种：Both species have distinct advantages — choose based on your experimental goals:

特征FeatureX. tropicalisX. laevis
基因组倍性Ploidy二倍体（2n=20）Diploid (2n=20)异源四倍体（2n=36）Allotetraploid (2n=36)
基因组大小Genome size~1.7 Gb~3.1 Gb
世代周期Generation time4–5 个月months12–18 个月months
成体体长Adult size5–7 cm9–12 cm
适合温度Optimal temperature25–28 °C18–22 °C
CRISPR 编辑CRISPR editing更高效（二倍体，靶点唯一）More efficient (diploid, unique targets)存在基因冗余Gene redundancy present
卵母细胞产量Oocyte yield较少Lower极大（首选表达系统）Very large (preferred expression system)
主要优势领域Main strengths遗传学、基因组功能、正向遗传Genetics, genomics, forward screens发育生物学、卵母细胞表达、神经生物学Developmental biology, oocyte expression, neurobiology

主要应用领域Applications

  • 正向遗传筛选：二倍体基因组使隐性突变表型在 F2 代可直接显现，是 ENU 诱变或自发突变功能筛选的理想物种；世代周期短（4–5 月）可大幅提升筛选效率。Forward genetic screens: diploid genome allows recessive mutant phenotypes to appear in F2; short generation time (4–5 months) greatly accelerates screening.
  • CRISPR / Cas9 基因编辑：靶位点唯一（二倍体），脱靶效应评估更简单，编辑效率更高；适合通过注射 Cas9 mRNA + sgRNA 快速建立基因功能缺失模型。CRISPR/Cas9 gene editing: unique target sites (diploid), simpler off-target assessment, higher efficiency; suitable for rapid loss-of-function models via Cas9 mRNA + sgRNA injection.
  • 发育生物学：胚胎发育规律与 X. laevis 高度相似，NF 分期完善，适合组织归宿图谱（fate mapping）、信号通路功能验证。Developmental biology: embryonic development closely parallels X. laevis; complete NF staging suitable for fate mapping and signalling pathway validation.
  • 基因组功能研究：对应人类直系同源基因分析更为清晰（相比于四倍体 X. laevis 减少了冗余），Xenbase 等数据库对其基因组注释完善。Genomic functional studies: human orthologue analysis is cleaner without tetraploid redundancy; well-annotated in Xenbase and other databases.

供应与饲养说明Supply & husbandry notes

  • 每批次提供检疫报告与健康证明文件。Each batch comes with quarantine report and health certification.
  • 附赠热带爪蟾专用养殖指南（温度、水质、饲料与繁殖方案）。Includes dedicated X. tropicalis care guide (temperature, water quality, feed and breeding protocols).
  • 如需配对产卵实验，请提前告知，中心可协助制定 HCG 注射诱导排卵方案。For planned spawning experiments, contact us in advance — we can help design HCG-injection ovulation protocols.
  • 到货 48 小时内死亡，凭照片补发或退款。Deaths within 48 h of arrival are replaced or refunded with photographic evidence.

其他供应Other supply

成年野生非洲爪蟾

成年野生非洲爪蟾Adult wild-type X. laevis
白化非洲爪蟾

白化非洲爪蟾Albino X. laevis
爪蟾专用饲料

爪蟾专用营养饲料Specialized frog feed