基于标准化实验流程与严格质控体系,提供从单细胞操作、显微注射到蛋白功能检测的全流程爪蟾实验技术服务。客户成果已发表于 Nature、Cell Research、Neuron 等期刊。Full-workflow Xenopus services — from single-cell manipulation and microinjection to protein-function assays. Client work has appeared in Nature, Cell Research, Neuron and more.
量身设计实验方案,含昆虫嗅觉/信息素受体等特殊蛋白的定制表达与功能鉴定。Tailored plans, incl. custom expression of insect olfactory/pheromone receptors.
早期胚胎单卵裂球分离,用于谱系追踪、发育信号与单细胞分析。Blastomere isolation for lineage tracing, developmental signaling and single-cell analysis.
离子通道、转运蛋白与受体的异源表达与 TEVC 功能检测(主力服务)。Heterologous expression and TEVC for ion channels, transporters and receptors.
环境因子与化合物的发育毒性、致畸与致死评价(FETAX 思路)。Developmental toxicity, teratogenicity and lethality (FETAX-style).
低渗溶胀法精准测定水通道蛋白(Aquaporin / PIP)Pf 值,同步共聚焦膜定位验证。Hypotonic swelling assay for aquaporin (AQP/PIP) Pf, with optional confocal localization.
¹³C 同位素示踪(LC-MS)+ 荧光探针两种策略,测定 Km、Vmax、IC50,覆盖植物、动物、微生物转运蛋白。¹³C stable-isotope tracing (LC-MS) + fluorescent probes; Km, Vmax, IC50 for plant, animal and microbial transporters.
离子通道 I-V 关系、动力学参数(τact/τinact)、药理学响应(IC50/EC50)全套电生理数据。Full I-V relationship, kinetics (τact/τinact), pharmacology (IC50/EC50) electrophysiology report.
六大模块系统培训:卵母细胞分离、分子克隆、cRNA 注射、TEVC 记录、胚胎操作与养殖管理,小班手把手指导。Six-module training: oocyte isolation, cloning, cRNA injection, TEVC recording, embryo manipulation and husbandry — small-group hands-on instruction.
描述你的实验目标,我们提供方案、报价与时间安排。Describe your goal — we'll send a plan, quote and timeline.