技术服务Technical Services

基于标准化实验流程与严格质控体系,提供从单细胞操作、显微注射到蛋白功能检测的全流程爪蟾实验技术服务。客户成果已发表于 Nature、Cell Research、Neuron 等期刊。Full-workflow Xenopus services — from single-cell manipulation and microinjection to protein-function assays. Client work has appeared in Nature, Cell Research, Neuron and more.

标准化体系Standardized

规范流程 · 严格质控Defined workflow · Strict QC

每一项服务都遵循统一操作规范与质控节点,确保实验的精准性与可重复性,数据可靠、结果可信。Every service follows unified SOPs and QC checkpoints — precision, reproducibility, reliable data.

1
需求沟通Consult
2
方案设计Design
3
实验执行Run & QC
4
交付报告Deliver
mRNA/cRNA注射与TEVC检测流程
专业订制服务
01 · 定制Custom

专业订制服务Custom services

量身设计实验方案,含昆虫嗅觉/信息素受体等特殊蛋白的定制表达与功能鉴定。Tailored plans, incl. custom expression of insect olfactory/pheromone receptors.

02 · 单细胞Single-cell

早期胚胎单细胞提取Single-cell isolation

早期胚胎单卵裂球分离,用于谱系追踪、发育信号与单细胞分析。Blastomere isolation for lineage tracing, developmental signaling and single-cell analysis.

早期胚胎单细胞
mRNA/cRNA注射与卵母细胞表达
03 · mRNA / cRNA · TEVC

注射与卵母细胞蛋白表达Injection & oocyte expression

离子通道、转运蛋白与受体的异源表达与 TEVC 功能检测(主力服务)。Heterologous expression and TEVC for ion channels, transporters and receptors.

04 · 毒理Toxicology

蝌蚪胚胎发育毒性实验Embryonic toxicity testing

环境因子与化合物的发育毒性、致畸与致死评价(FETAX 思路)。Developmental toxicity, teratogenicity and lethality (FETAX-style).

蝌蚪胚胎发育毒性
膜渗透率检测服务
05 · 渗透率Permeability

卵母细胞膜渗透率检测Membrane water permeability assay

低渗溶胀法精准测定水通道蛋白(Aquaporin / PIP)Pf 值,同步共聚焦膜定位验证。Hypotonic swelling assay for aquaporin (AQP/PIP) Pf, with optional confocal localization.

06 · 转运Transport

卵母细胞跨膜转运功能分析Transmembrane transport assay

¹³C 同位素示踪(LC-MS)+ 荧光探针两种策略,测定 Km、Vmax、IC50,覆盖植物、动物、微生物转运蛋白。¹³C stable-isotope tracing (LC-MS) + fluorescent probes; Km, Vmax, IC50 for plant, animal and microbial transporters.

跨膜转运功能分析
TEVC双电极电压钳检测
07 · TEVC

TEVC 双电极电压钳检测Two-electrode voltage clamp (TEVC)

离子通道 I-V 关系、动力学参数(τactinact)、药理学响应(IC50/EC50)全套电生理数据。Full I-V relationship, kinetics (τactinact), pharmacology (IC50/EC50) electrophysiology report.

08 · 培训Training

卵母细胞相关技术培训Oocyte technology training

六大模块系统培训:卵母细胞分离、分子克隆、cRNA 注射、TEVC 记录、胚胎操作与养殖管理,小班手把手指导。Six-module training: oocyte isolation, cloning, cRNA injection, TEVC recording, embryo manipulation and husbandry — small-group hands-on instruction.

技术培训服务

把实验交给专业的爪蟾平台Let our Xenopus platform run it

描述你的实验目标,我们提供方案、报价与时间安排。Describe your goal — we'll send a plan, quote and timeline.

获取实验方案Request a plan