Internal Saline Weight per 10 ml(mg)MwFinal Conc.(mM)Cs Meth Sul182.40228.090MgCl210.17203.35TEA-Cl33.14165.720EGTA38.04380.410HEPES47.66238.320ATP11.00551.12GTP1.57
2019-03-05 300
选择近半年内未取过卵母细胞的爪蟾,冰浴30-45min,待其四肢麻痹,翻正反射消失后,在其下腹部正中皮肤及内部肌肉处切一个0.5-1cm的小口,拉出宫瓣,剪出试验所需量的爪蟾卵母细胞,置于含OR2溶液(41mmol/LNaCl,1mmol/LKCl,0.5mmol/LMgCl2,2.5mmol/LHEPES)的培养皿中待用,缝合爪蟾的肌肉及皮肤,置于浅水中(注意使其头部露在水面上方),使其自然苏醒
2014-12-06 admin 841
MethodforBulkTissueElectroporationWehaveadaptedelectroporationfortransferofmacromolecules,includingDNAintomanyneuronsinthetadpolebraininvivo(K.Haasetal.,2002).BulkelectroporationpermitsonetotargetDNAt
2014-06-01 admin 591
IntroductionSingle-cellelectroporation(SCE)isatechniquewehavedevelopedtodelivergenesintoindividualcellswithinintacttissues(K.Haasetal.,2001;K.Haasetal.,2002;J.E.Bestmanetal.,2006),althoughitisalsobeap
2014-06-01 admin 556