白化非洲爪蟾（科研全阶段供应）Albino Xenopus laevis — all developmental stages

白化品系的科研优势Why the albino line?

野生型非洲爪蟾皮肤含大量黑色素，在荧光显微镜下会产生强烈的自发荧光背景，对 GFP、mCherry 等荧光报告基因的观察造成干扰。白化品系因缺乏酪氨酸酶功能而不能合成黑色素，整体体色浅淡或接近白色：Wild-type Xenopus laevis skin contains abundant melanin, generating strong autofluorescence background that interferes with GFP, mCherry and other fluorescent reporter observations. The albino strain lacks functional tyrosinase and cannot synthesise melanin, resulting in pale or near-white colouration:

• 胚胎与蝌蚪期：整个蝌蚪体壁透明度高，可在活体状态下直接通过解剖镜或共聚焦显微镜观察大脑（视顶盖）发育、内脏形态、血液循环和神经束走向，无需组织切片。Embryo & tadpole stages: High body-wall transparency allows direct in-vivo observation of brain (optic tectum) development, visceral morphology, blood circulation and nerve tracts through a stereomicroscope or confocal — no sectioning required.
• 成体期：皮下色素稀少，特别适合对腺体、卵巢或皮下植入物进行外部观察，也更易进行经皮荧光检测实验。Adult stage: Minimal subdermal pigment makes it easier to observe glands, ovaries or subcutaneous implants externally, and to perform transcutaneous fluorescence assays.
• 卵母细胞：白化型卵母细胞动物极色素浅，注射后更易在荧光体式镜下判断注射成功与否，且适合荧光探针功能实验。Oocytes: Lightly pigmented animal pole makes injection success easier to confirm under a fluorescence stereomicroscope, and is better suited for fluorescent probe assays.

主要应用领域Applications

• 发育生物学：Morpholino 敲低、mRNA 过表达引发的形态学表型在透明蝌蚪中可直接活体记录，省去整体染色步骤。Developmental biology: Morpholino knockdown or mRNA overexpression phenotypes can be recorded live in transparent tadpoles, eliminating whole-mount staining steps.
• 荧光细胞标记与示踪：注射荧光蛋白 mRNA 或荧光染料后，在白化背景下信噪比显著提升，适合长时程活体成像。Fluorescent cell labelling & tracing: Injection of fluorescent protein mRNA or dyes yields markedly higher signal-to-noise ratio against the albino background, ideal for long-term live imaging.
• 药物毒性与代谢动态：荧光标记药物在白化蝌蚪体内的分布、代谢与排泄可全程活体追踪，用于环境污染物生物积累研究及药物安全性评估。Drug toxicity & metabolism: Fluorescently labelled drugs can be tracked in real time through distribution, metabolism and excretion in live albino tadpoles — useful for environmental pollutant bioaccumulation and drug safety studies.
• 基因功能研究：注射 CRISPR-Cas9 mRNA + sgRNA 进行快速基因编辑，表型可在 F0 蝌蚪中即时观察，加速功能验证。Gene function research: CRISPR-Cas9 mRNA + sgRNA injection for rapid gene editing; phenotypes observable immediately in F0 tadpoles, accelerating functional validation.
• 组织再生研究：截肢或视神经损伤再生过程中的细胞迁移、轴突生长可在白化蝌蚪中实时追踪。Tissue regeneration: Cell migration and axon regrowth during limb or optic nerve regeneration can be tracked in real time in albino tadpoles.

供应与订购说明Supply & ordering notes

• 受精卵与早期胚胎需提前预约（需配合排卵周期，建议提前 2 周告知需求日期）。Fertilised eggs and early embryos must be pre-arranged (synchronized to ovulation cycle — notify us at least 2 weeks ahead).
• 蝌蚪可按 NF 分期（如 NF 40–50、NF 51–56 等）定制供应，请说明实验所需分期范围。Tadpoles can be ordered by NF stage (e.g., NF 40–50, NF 51–56); please specify the required stage range.
• 成体按性别可选，雌蛙可提供产卵史记录，已验证产卵质量。Adults are available by sex; females can include a spawning history with verified egg quality records.
• 全程冷链控温包装，充纯氧，运输中存活率 ≥ 95%；到货如有死亡请在 48 小时内拍照联系，按约定补发。Cold-chain oxygenated shipping; ≥95% survival guaranteed — report any deaths with photos within 48 hours and we will arrange replacements.
• 可配套提供爪蟾专用饲料、养殖用具与实验操作手册。Specialized feed, husbandry supplies and experimental operation manuals available as add-ons.