爪蟾实验技术要点与养殖管理经验，帮助科研人员更高效地开展爪蟾相关研究。Key Xenopus techniques and husbandry know-how to run frog-based research more efficiently.
手术取卵、胶原酶处理、去卵泡及 V–VI 期挑选与维护。Retrieval, collagenase, defolliculation, stage V–VI selection.
注射针拉制、体积标定与注射量控制。Needle pulling, volume calibration and dose control.
电极充灌、灌流系统与电压方案设计。Electrode filling, perfusion and voltage protocols.
体外受精、去胶、卵裂球注射与 NF 分期。IVF, dejellying, blastomere injection and NF staging.
| 参数Parameter | 推荐范围Recommended |
|---|---|
| 水温Water temp. | 18–22 °C |
| pH | 6.5–7.5 |
| 光周期Photoperiod | 12 h 光 / 12 h 暗12 h L / 12 h D |
| 换水Water change | 定期部分换水，去氯Partial, dechlorinated |
| 投喂Feeding | 专用蛙粮，每周 2–3 次Pellets, 2–3×/week |
| 诱导排卵Ovulation | hCG 注射诱导hCG-induced |